Conclusion Constructing the replacement vector which is used to the gene knockout in BCG. constructing the BCG mutation with deleted ERP gene. 结论构建了卡介苗ERP基因置换型打靶载体,并构建筛选卡介苗ERP基因缺失突变株。
Conclusion: Constructing successfully the replacement vector which is used to the gene knockout in Mycobacterium Tuberculosis. 结论:成功构建了用于结核分支杆菌基因打靶的置换型载体,为随后将进行的Rv0901基因敲除株的建立,Rv0901基因功能的研究奠定了基础。
Construction and application of gene targeting replacement vector 基因打靶置换型载体的构建和应用研究